Corrigendum: Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins
نویسندگان
چکیده
This Article contains an error in the Methods section under subheading 'Freeze substitution, resin embedding and sectioning'. " The first 12 h of polymerization were done at − 45 °C, during which the samples were covered with foil and indirectly exposed to UV light. The cover was then removed and samples were warmed to 0 °C at 5 °C/h and polymerized for a further 8 h, then left in the fumehood at room temperature for 1–2 days. " should read: " The first 12 h of polymerization were done at − 45 °C, during which the top of the samples were covered with foil and indirectly exposed to UV light. The cover was then removed and samples were polymerized for a further 12 h at −45 °C, then warmed to 0 °C over a 12 h period and polymerized at 0 °C for a further 12 h. Samples were then left in the fume hood at room temperature for 1–2 days. " This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
منابع مشابه
Correlative in-resin super-resolution and electron microscopy using standard fluorescent proteins
We introduce a method for correlative in-resin super-resolution fluorescence and electron microscopy (EM) of biological structures in mammalian culture cells. Cryo-fixed resin embedded samples offer superior structural preservation, performing in-resin super-resolution, however, remains a challenge. We identified key aspects of the sample preparation procedure of high pressure freezing, freeze ...
متن کاملCorrelative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo
Super-resolution light microscopy, correlative light and electron microscopy, and volume electron microscopy are revolutionising the way in which biological samples are examined and understood. Here, we combine these approaches to deliver super-accurate correlation of fluorescent proteins to cellular structures. We show that YFP and GFP have enhanced blinking properties when embedded in acrylic...
متن کاملultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy
In-resin fluorescence (IRF) protocols preserve fluorescent proteins in resin-embedded cells and tissues for correlative light and electron microscopy, aiding interpretation of macromolecular function within the complex cellular landscape. Dual-contrast IRF samples can be imaged in separate fluorescence and electron microscopes, or in dual-modality integrated microscopes for high resolution corr...
متن کاملCorrelative Photoactivated Localization and Scanning Electron Microscopy
The ability to localize proteins precisely within subcellular space is crucial to understanding the functioning of biological systems. Recently, we described a protocol that correlates a precise map of fluorescent fusion proteins localized using three-dimensional super-resolution optical microscopy with the fine ultrastructural context of three-dimensional electron micrographs. While it achieve...
متن کاملCorrelative light- and electron microscopy with chemical tags.
Correlative microscopy incorporates the specificity of fluorescent protein labeling into high-resolution electron micrographs. Several approaches exist for correlative microscopy, most of which have used the green fluorescent protein (GFP) as the label for light microscopy. Here we use chemical tagging and synthetic fluorophores instead, in order to achieve protein-specific labeling, and to per...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 6 شماره
صفحات -
تاریخ انتشار 2016